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KMID : 0363020050350040939
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 4 p.939 ~ p.948
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Role of the periosteum on bone regeneration in rabbit calvarial defects
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장현성
김상목/박주철/김병옥
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Abstract
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The role of the periosteum on osteointegration of Bio-Ossⓡ(Geistlich, Wolhusen/Switzerland) was
studied in rabbit calvarial defect. 12 New Zealand white male rabbits between 2.8 and 4 kg
were included in this randomized, blinded, prospective study. Each rabbit was anesthetized
with Ketamine HCl(5 ㎎/㎏) and Xylazine HCl(1.5 ㎖/㎏). An incision was made to the bony
cranium and the periosteum was reflected. Using a 6-㎜ trephine bur(3i. USA), four 8-㎜defects were created with copious irrigation. The defects were classified into barrier
membrane(Tefgenⓡ, Lifecore Biomedical, Inc, U.S.A.) only group as a control, Bio-Ossⓡ with barrier
membrane group, Bio-Ossⓡ with periosteum covering group, and Bio-Ossⓡ without periosteumcovering group. There were 2 rabbits in each group. The wound was closed with resorbable
suture materials. Rabbits were sacrificed using phentobarbital(100 ㎎/㎏) intravenously at 1, 2,
and 4 weeks after surgery. The samples were fixed in 4% paraformaldehyde, and decalcified in
hydrochloric acid decalcifying solution(Fisher Scientific, Tustin, CA) at 4℃ for 2-4 weeks. It was
embedded in paraffin and cut into 6 ㎛ thickness. The sections were stained with H & E and
observed by optical microscope.
The results were as follows; 1. The periosteum played an important role in osteointegration
of Bio-Ossⓡ in bone defects. 2. When the periosteum remained intact and Bio-Ossⓡ was placed
on the defect, Bio-Ossⓡ with periosteum covering has been incorporated into the newly formed
bone from 2-week postoperatively. 3. When the periosteum was removed at the surgical
procedure, invasion of connective tissue took place among the granules, and new bone
formation was delayed compared to periosteum covering group.
Therefore, when the bone grafting was performed with periosteal incision procedure to
achieve tension-free suture, the integrity of the overlying periosteum should be maintained to
avoid fibrous tissue ingrowth.2)
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KEYWORD
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periosteum, bone regeneration
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